How do Hsp90 genetic interaction networks change over time?
Hsp90 regulates about 4% of the Candida albicans genome (Diezmann et al., 2012). While this is comparable to its close relative, the eukaryotic model organism Saccharomyces cerevisiae, both Hsp90 networks have substantially diverged since the yeasts split from their most common ancestor some 300-400 million years ago (Stajich et al, 2009).
This project investigates how networks change over time and in response to the selective forces exerted by the yeasts’ distinct ecological niches. To determine the role of evolution and ecology on Hsp90 genetic interaction networks we are mapping the fungal chaperone network landscape employing ecologically distinct yeast species, which diverged more than 500 million years ago. To understand how environmental fluctuations, yeast population structure, and time affect network dynamics, networks will be analyzed quantitatively. We will furthermore test specific Hsp90 genetic interactors for their contribution to phenotypic variation in the context of virulence and stress response.